Confocal Raman Mircroscope

WITec's AutoBeam concept for automatic and motorized beam path alignment includes:

• Motorized 6-position objective turret: positions selected objective and compensates offsets
• Motorized illumination selector: switches between all microscopy illumination options
• Motorized laser coupler: delivers up to 6 laser wavelengths from UV to NIR
• AutoBeam output coupler: automatically optimizes signal & resolution and selects spectrometers
• Motorized calibration source: fast & automatic multi-point spectrometer calibration over the full spectral range
• Motorized Köhler illumination apertures: facilitate focusing and optimize contrast and homogeneity
• Motorized polarization modules: freely rotatable automated excitation polarizer and detection analyzer

WITec'sTruePower sets and determines absolute laser power with <0.1 mW accuracy.

• Instrument (re-)positions sample automatically with motorized and piezo-driven scan stages.
• Focus stabilization finds and actively maintains focus during the entire measurement.
• Motorized objective turret includes precise offset-compensation.
• EasyLink handheld controller offers intuitive instrument operation.

WITec'sSuite SIX software wizard guides the user through the processing of Raman spectra, from background reduction through image generation.

• TrueComponent Analysis: automatically locates the sample components in an image and differentiates their individual spectra.

WITec's Apyron system provides sharp 2D and 3D images with resolution limited only by physical law:

• lateral resolution <300 nm (typically at 532 nm excitation).
• depth resolution <900 nm (typically at 532 nm excitation).
• yields spectral resolution down to 0.1 cm-1 relative wavenumbers (at 633 nm excitation).
• supports ultra-fast Raman imaging without sacrificing resolution (acquisition speed <1ms per spectrum).

WITec's TrueSurface module:

• The optical sensor controls the distance between the objective and the sample surface with sub-micrometer precision. Thus, any variation occurring during measurements with long integration times is compensated for, resulting in perfectly sharp and detailed images.
• Multi-spectral searches enable the user to identify several spectra simultaneously, using databases to return hit rates describing the probability of a match for each individual spectrum:

WITec's TrueMatch software identifies molecules by comparing measured spectra with existing databases:

• Simultaneous multi-spectra search (1:1 match)
• Multiple component search: combine up to three database components to describe a measured composite spectrum
• Automated and simultaneous demixing analysis of multiple components
• TrueMatch Databases
• Rely on existing S.T. Japan database (>24,000 spectra) or create your own catalog of spectra.

WITec's ParticleScout:

• Integration time optimization – reduces measurement time and minimizes the effects of fluorescence.
• Vignetting correction – ensures uniform brightness throughout the image plane.
• Smart zoom – displays particle information dynamically depending on viewed area.
• Sample area targeting – allows the selection of multiple regions of interest for each investigation.
• Dark-field, bright-field, epifluorescence and transmission sample illumination – provides the best option for each type of particle.
• Round sample wedge sectioning – allows the selection of an area for data acquisition, typically on a filter, that can be extrapolated to represent the whole.
• Smart separation of particles – differentiates materials in densely packed, heterogeneous samples.
• Quantitative report formatting – presents data using table, bar graph histogram and pie chart templates for ease and clarity.

Oxford Instruments Microstat Hi Res 2 cryostat with the following specifications:

• Temperature range 2.7K – 500K:
• 3.2 to 500 K with GF4 pump
• 2.7 to 500 K with EPS40 pump
• Temperature stability: ± 0.1 K using an Oxford Instruments MercuryITC temperature controller (measured over a 10 min period)
• Liquid helium consumption rate at 4.2 K: < 0.7 litre/hr
• Cryostat cool down time from room temperature to base temperature: approx. 15 min with pre-cooled transfer tube
• Cool down consumption: approx. 0.8 litres
• Lateral sample drift at a constant temperature of 4.2 K (typical): 0.15 μm per hour
• Lateral sample drift on cooling from 300 to 4.2 K (typical): 13.0 μm
• Sample working distance minimum sample holder surface to window outer surface:
• 2.2 mm with 0.5 mm thick windows fitted
• 3.2 mm with 1.5 mm thick windows fitted
• Adjustable distance of top flange: 3.5 mm
• Sample holder diameter: 20 mm
• Heat exchanger fitted with heater and rhodium iron temperature sensor
• Blanked off port for customer wiring
• Cryostat weight (including QOVC05HR2 and VH4HR2): approx. 1.5 kg
• System may also be run with liquid nitrogen temperature range 77 to 500 K

The OI Microstat cryo-head includes:

• Adapter to WITec Confocal Raman Imaging System with motorized or manual sample stage including 2x10mm spacers to elevate Microscope
• Transmission sample holder for up to 4 mm thick samples 5 mm diameter aperture
• Sample holder for up to 4 mm thick samples
• LX10 ten pin electrical connector wired to terminals above sample holder
• Cryostat top plate with 1.5 mm thick 25 mm diameter optical access Spec. B fused quartz window
• Cryostat base plate with 1.5 mm thick 25 mm diameter optical access Spec. B fused quartz window
• Mercury ITC temperature controller configured with one PID loop