Title: Evolution of operon formation in the laboratory

Presenter: Mr. Yuki Kanai, PhD student

Affiliation: Department of Biological Sciences, University of Tokyo, Japan

Abstract:

Operons are a fundamental feature of prokaryotic gene regulation. However, how they form through evolution is yet to be understood. Through an attempt to mimic the genome evolution of bacteria living in insects, we came up with a new model of operon formation: mobile genetic elements called insertion sequences (IS) brings together two genes placed apart to form operons. We constructed a fluorescent reporter system, introduced it into Escherichia coli, and evolved it in the laboratory, showing that new operons can form by the proposed mechanism. Our study implies that genomes of modern prokaryotes have been shaped through evolution under coexistence with mobile genetic elements.  

Publication Link:

https://academic.oup.com/nar/article/50/3/1673/6511971

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Zoom:
https://oist.zoom.us/j/91251870346?pwd=cUkvbWNvUGkrMlZXRmI5ZTQxOEJvZz09

Meeting ID: 912 5187 0346
Pass code: 328577