csu FY2019 Annual Report 3.3 Figure 1

Figure 1. Cnot1-KO MEFs undergo cell death. (A) Lysates were prepared from Cnot1-flox MEFs that were infected with mock or Cre-expressing retrovirus and subjected to immunoprecipitation with anti-CNOT3 antibody. CNOT3 are shown in red to indicate a precipitated molecule. Lysates and immunoprecipitates (IP) were analysed by immunoblot with the indicated antibodies. (B) Morphology of Cnot1-flox MEFs infected with mock (Control) or Cre-expressing retrovirus (Cnot1-KO). Photographs are at the same magnification and represent one of the three independent experiments. Dead cells that were about to lose adhesion were observed in Cnot1-KO MEFs. (C) Cell death was assessed by propidium iodide uptake using flow cytometry (n = 3).