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Figure 3.1.2: Model-based prediction of A2AR-stimulated phosphoresponse with permeation of agonists into slice preparations. (A) Time course of estimated effective concentration in a slice preparation of CGS21680. (B) Simulated traces of phosphorylation of GluR1 (Ser845). Blue and red lines are the simulated phosphoresponses by the original kinetic signal transduction model and by the model with assumption of permeation delay of CGS21680 solution, respectively. Black squares are experimental observations. (c) CGS21680 dose-response curves for six different agonist concentrations.